名称→vonWillebrandFactor(VWFR9.14)类型→2'-F-RNA靶标→vonWillebrandFactor(VWF)靶标类别→蛋白质

名称：   

vonWillebrandFactor(VWFR9.14)

类型：    

2'-F-RNA

靶标：    

vonWillebrandFactor(VWF)

靶标类别：    

蛋白质

KD：    

12nM(reportedvalue)

缓冲液：    

 For nitrocellulose filter binding assays E (20mM HEPES (pH 7.4); 50 mM NaCl; 2 mM CaCl2; and 0.1% BSA) -OR- F (20mM HEPES (pH 7.4); 150 mM NaCl; 2 mM CaCl2; and 0.1% BSA) For platelet function analysis(performed at RT) Platelet binding buffer150 mM NaCl; 20 mM Hepes pH 7.5; 5 mM KCL; 1 mM MgCl2; and 1 M CaCl2.

重折叠条件：    

提醒：    

Binds to the A1 domain of vWF and completely inhibits ristocetin-induced platelet aggregation at a concentration of 250 nM. Inhibition was reversed using complementary sequence: GGTUCCTCAGCGAGUCGUCUG

序列：    

5'GGGAGGAfCpGAfUpGfCpGGfUpGGAfCpGAAfCpfUpGfCpfCpfCpfUpfCpAGfCpfUpAfCpfUpfUpfUpfCpAfUpGfUpfUpGfCpfUpGAfCpGfCpAfCpAGAfCpGAfCpfUpfCpGfCpfUpGAGGAfUpfCpfCp

长度：    

76

分子量（g / mol）

24563.62

消光系数L /（mol·cm）：    

731400

GC含量：    

59.21%

OD260：

1.37 μg/OD260: 33.58

参考文献：    

S Oney; BA Sullenger; et al. (2007) Antidote-Controlled Platelet Inhibition Targeting von Willebrand Factor with Aptamers. Oligonucleotides; 17:265-274.